Electrophoretic studies of immune rabbit sera

Leukocyte antibodies in human sera and in immune rabbit sera.

An Electrophoretic Study of Immune Sera and Purified Antibody Preparations

1. Antibody produced in the horse migrates as a new serum component between the beta and gamma components, whereas rabbit antibody is electrophoretically identical with the gamma globulin component of the serum. 2. In rabbit and monkey antisera the percentage of antibody in the serum and in the gamma globulin fraction can be determined by integration of the electrophoresis diagrams of unabsorbe...

Electrophoretic Study of Antiviral Sera

1. Sera of animals immunized against Japanese B encephalitis, Venezuelan equine encephalomyelitis, and Western equine encephalomyelitis viruses were fractionated by electrophoresis. 2. Electrophoretic patterns of rabbit sera before and after immunization against Japanese B virus showed no consistent change traceable to antibody formation. 3. To determine the antibody content, the electrophoreti...

AN ELECTROPHORETIC STUDY OF IMMUNE SERA AND PURIFIED ANTIBODY PREPARATIONS*t

Studies on the eleetrophoresis of serum have shown that besides the albumin normal sera possess three separate globulin components differing in mobility and designated as cz, /3, and ~' (1). The relationship of antibodies to these components is of considerable importance. Differences in the antibody globulin formed in various animal species have already been found by ultracentrifugal studies (2...

Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera.

Serodiagnosis of cytomegalovirus (CMV) infection by complement fixation tests depends on showing a fourfold rise in antibody titer from acute- to convalescent-phase sera. Freeze-thaw and glycine-extracted, infected cell culture antigens used for these tests give markedly different titers in reactions with the same sera. In this study, we characterized the CMV-infected cell polypeptides containe...